【摘要】 对映-贝壳杉烷型二萜是药用植物香茶菜抗菌抗肿瘤的核心组分,其生物合成的酶分子机制尚未完全明了。前期我们克隆并鉴定了毛萼香茶菜二萜合酶柯巴基焦磷酸合酶基因Ie CPS1和Ie CPS2。本文通过与拟南芥对映-贝壳杉烯二萜合酶At KS配对进行偶联反应,以及GC-MS鉴定酶联反应产物,证实毛萼香茶菜二萜合酶Ie CPS1和Ie CPS2的立体特异性为对映型(ent-CPS)。更多还原

【Abstract】 Ent-kaurane diterpenoids, isolated from the Chinese medicinal herbs, Isodon L., are principle components showing potent bioactivities of antitumor and anti-autoimmune infl ammation. Despite a large number of ent-kaurane diterpenoids isolated from Isodon plants, little is known about the enzymatic machinery involved in their biosynthesis. Ie CPS1 and Ie CPS2 encoding copalyl diphosphate synthases(CPS) were previously cloned from the I. eriocalyx leaves and functionally characterized. Here we verified that Ie CPS1 and Ie CPS2 were ent-CPSs that produced CPP of ent-stereochemistry, by GC-MS analysis of enzymatic products from coupled reactions with a known Arabidopsis ent-kaurene synthase stereospecifi c for ent-CPP.更多还原