In this paper, we studied in vitro culture of Bredia amoena by using leaf explants, including callus induction, adventitious shoot differentiation and plantlet regeneration. The results showed that the best medium for callus induction and adventitious shoot differentiation was MS+6-BA 1.0 mg·L~(-1)+2,4-D 0.3 mg·L~(-1), with an induction rate of 100% and a differentiation rate o f 85.83%. The best multiplication medium was MS+6-BA 1.0 mg·L~(-1)+IBA 0.3 mg·L~(-1), the multiplication coeffi cient reached 7.38,...