【摘要】 目的利用超高效液相色谱技术,建立保健食品中木香烃内酯和去氢木香烃内酯含量的检测方法。方法样品经甲醇提取,采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),以乙腈-水(40∶60,V/V)为流动相,紫外检测器波长为225 nm,流速为0.5 ml/min,进样体积为5μl,柱温为30℃,通过超高效液相色谱分离,以外标法定量。结果木香烃内酯和去氢木香烃内酯的浓度为0μg/ml~1 000μg/ml时,标准曲线的线性关系良好,线性相关系数为0.999 9。相对标准偏差(RSD)为0.5%~3.7%,加标回收率分别为96.2%~100.6%、96.2%~103.9%。结论本方法具有灵敏度高、选择性好、精密度和准确度好等优点,而且更快捷,适合保健食品的日常检测工作。更多还原

【Abstract】 Objective To establish an ultra high performance liquid chromatography( UPLC) method for the determination of costunolide and dehydrocostus lactone in health food. Methods Samples were extracted with methanol,and seperated by UPLC and quantified with external standard method under the experiment conditions of ACQUITY UPLC BEH C18( 2. 1 mm × 50 mm,1. 7 μm),acetonitrile- water( 40 ∶60,V / V) as mobile phase,the UV detector wavelength was 225 nm,the flow rate was0. 5 ml / min,the sample volume was 5 μl,and the column temperature was 30 ℃. Results When the concentration of costunolide and dehydrocostus lactone were among 0 μg / ml- 1 000 μg / ml,there was good linearity,and the correlation coefficent of the standard curve( r) was 0. 999 9. The relative standard derivation( RSD) was among 0. 5%- 3. 7%,and the recoveries were 96. 2%- 100. 6% and 96. 2%- 103. 9%,respectively. Conclusion The methodis rapid,sensitive,selective,with excellent precision and accuracy. It can be used for the routine detection in health food.更多还原