【摘要】 目的探讨联合检测血清中抗环瓜氨酸肽抗体(抗-CCP)、抗角蛋白抗体(AKA)与类风湿因子(RF)对类风湿性关节炎(RA)的诊断意义。方法分别采用酶联免疫吸附法(ELISA)法、间接免疫荧光法和速率散射比浊法测定96例RA患者和40例健康体检者的抗-CCP、AKA、RF水平,比较3种抗体单独和联合检测对诊断RA的敏感度和特异度。结果 RA组中的抗-CCP、AKA和RF的阳性率分别为58.3%、40.6%和67.7%。对RA诊断的敏感度、特异度分别为:抗-CCP:67.7%、89.2%;AKA:47.5%、86.5%;RF:74.6%、73.2%。3项指标联合检测的敏感度、特异度分别为90.3%、91.6%。抗-CCP与AKA、RF之间显著相关(P<0.05)。结论抗-CCP、AKA及RF的联合检测可使类风湿性关节炎的诊断敏感度和特异度得到有效提高。更多还原

【Abstract】 Objective To assess the value of anti- cyclic citrullinated peptide( anti- CCP) antibody,anti- keratin antibodies( AKA) and rheumatoid factor( RF) for diagnosis of patients with rheumatoid arthritis( RA). Methods Ninety- six patients with RA and forty healthy physical examinees were enrolled in the study. Levels of serum anti- CCP,AKA and RF were detected by ELISA,indirect immunofluorescence assay and rate nephelometry,respectively. The sensitivity and specificity of the 3antibodies against the diagnosis of RA were compared. Results The positive rate of anti- CCP,AKA and RF in patients with RA were 58. 3%,40. 6% and 67. 7%,respectively. The sensitivity and specificity of anti- CCP,AKA,RF for the diagnosis of RA were 67. 7% and 89. 2%,47. 5% and 86. 5%,74. 6% and 73. 2%,respectively. The sensitivity and specificity of combined detection of the three antibodies were 90. 3% and 91. 6%,and there was a significant correlation between anti- CCP and AKA and RF( P < 0. 05). Conclusion The combined detection of anti- CCP,AKA and RF can improve the diagnostic sensitivity and specificity of RA.更多还原