【摘要】 目的揭示鸡血藤水提取物A.E.体外抗流感病毒A1的作用。方法采用细胞病变CPE法测定鸡血藤水提取物A.E.在MDCK细胞的抗流感病毒的活性。同时采用血凝滴度测定鸡血藤水提物A.E.对鸡胚培养甲型流感病毒的抑制作用。小鼠经滴鼻感染流感病毒造成肺炎模型,观察其肺指数、死亡率及病理切片。结果鸡血藤水提物A.E.在MDCK细胞中对流感病毒A1具有一定的抑制作用,其半数抑制浓度IC50为(74.5±4.7)μg/ml,治疗指数TI为13.3。在鸡胚中与病毒对照组比较,鸡血藤水提物A.E.各剂量组的血凝滴度降低,差异有统计学意义(P<0.01)。每日灌注鸡血藤水提物A.E.能够明显降低病毒感染小鼠的死亡率、肺指数、肺病理切片坏死和浸润的程度。结论鸡血藤水提物对流感病毒A1具有抑制作用。更多还原

【Abstract】 Objective To investigate the antiviral effects of the aqueous extract( A. E.) of Spatholobus suberectus against influenza virus A1. Methods The inhibitory effects of A. E. on influenza virus( A1) in MDCK cell was detected by cytopathic effect( CPE). And the inhibition of A. E. of Spatholobus suberectus on influenza virus in chicken embryo culture was observed by hemagglutination test in the terms of hemagglutination titer. The mice were infected by influenza virus( A1) in nasal,the lung inflaramation index,mortality rate and pathological section were observed respectively. Results A. E. showed inhibitory activities against the influenza virus( A1) in MDCK cell,the IC50 was 74. 5 μg / ml ± 4. 7 μg / ml and the TI was 13. 3. And compared with the virus control group,the hemagglutination titer of the A. E. group decreased,which was statistically significant( P < 0. 01). The mice treated daily with A. E. after inoculation with influenza virus( A1),the mortality,LW / BW,necrosis and infiltration of the pathological sections were significantly decreased compared with the infected controls. Conclusion The extract of Spatholobus suberectus( A. E.) has inhibitory effect on influenza virus( A1).更多还原