【摘要】 目的:观察右美托咪定对神经病理性痛大鼠脊髓P2X4R总蛋白及膜蛋白表达水平的影响。方法:雄性SD大鼠40只,180~220 g,随机分为4组(每组10只):正常组(N组)、假手术组(S组)、神经病理性痛组(SNI组)、右美托咪定组(Dex组)。N组不作任何处理,Dex组于术后即刻腹腔注射右美托咪定40μg/kg,间隔24 h追加给药一次至术后14 d,S组和SNI组均腹腔注射等容量的生理盐水。术前1 d及术后1、3、5、7、14 d检测机械缩足反射阈值(MWT)。术后14 d用Western blot方法检测脊髓L4~6节段P2X4R总蛋白和膜蛋白表达量。结果:与N组和S组相比,SNI组大鼠术后MWT明显降低(P<0.05),脊髓水平P2X4R总蛋白和膜蛋白表达量均明显增加(P<0.05);与SNI组相比,Dex组MWT明显升高(P<0.05),脊髓水平P2X4R总蛋白和膜蛋白表达量均明显降低(P<0.05)。结论:Dex能减轻大鼠神经病理性痛,其机制可能与抑制脊髓P2X4R总蛋白及膜蛋白水平有关。更多还原

【Abstract】 Objective: To investigate effects of dexmedetomidine on neuropathic pain(NP)and expression of P2X4 R protein in the spinal cord in rats with NP. Methods: Forty male SD rats weighing 180 ~ 220 g were randomly divided into 4 groups(n = 10 in each group), they were naive(N), sham-operated(S), neuropathic pain(SNI)and dexmedetomidine-treated groups(Dex)respectively. N group rats received no treatment. In Dex group, Dexmedetomidine(40 μg/kg) was injected intraperitionally daily for 14 days starting immediately after surgery. S and SNI groups rats were injected saline as control. The mechanical paw withdrawal threshold(MWT) were measured before surgery and on postoperative day 1, 3, 5, 7 and 14. The L4~6 segment of spinal cords were used to determinate the level of P2X4 R total protein and membrane protein by Western blot on day 14. Results: MWT were significantly decreased(P < 0.05) and the level of expression of P2X4 R total protein and membrane protein increased significantly in the spinal cord in SNI rats, compared with those in N group and S group(P < 0.05). Compared with SNI group, MWT were significantly increased and the expression of P2X4 R total protein and membrane protein were significantly down-regulated after operation in Dex group(P < 0.05). Conclusion: Dexmedetomidine can reduce neuropathic pain in rats with SNI and its underlying mechanism may be involved in inhibition of expression of P2X4 R.更多还原