【摘要】 目的观察T3对小鼠糖尿病肾组织中转化生长因子β1(TGF-β1)表达的影响,探讨T3影响糖尿病肾组织纤维化的作用机制。方法 36只小鼠随机挑选12只为正常对照组(C),余24只以注射链尿佐菌素(STZ)方式复制糖尿病小鼠模型,分为糖尿病组(DM)和T3组(T3)。动物饲养6个月后取材肾组织,应用免疫组化技术、体视学方法(检测体密度)和Western blot技术观察各组肾组织中TGF-β1的表达。结果与对照组相比,糖尿病组小鼠TGF-β1的表达范围明显增加,T3组TGF-β1的表达范围明显低于糖尿病组。结论 T3通过降低TGF-β1影响糖尿病肾组织纤维化范围大小和进程。更多还原

【Abstract】 Objective To observe the effect of triiodothyronine( T3) on the expression of transforming growth factor- β1( TGF- β1),in order to investigate the mechanism of T3 in renal fibrosis.Methods Thirty-six C57/B6 mice were used in this study. The control group( C) included 12 mice,and the others were used to establish diabetic models by injection of streptozotocin( STZ). Then the mice were randomly divided into the diabetes group( DM) and T3 group( T3). The mice were raised for six months. The expression of TGF- β1was detected by immunohistochemical stainning,image anal-ysis and Western blot. Results Compared with the control group,the expression of TGF- β1was obviously increased in the diabetes group,and the expression of TGF- β1in the T3 group was obviously lower than that in the diabetes group. Conclusion T3 reduces the range and process of fibrosis in diabetic nephropathy by down-regulating expressions of TGF- β1.更多还原