【摘要】 目的检测系统性红斑狼疮(SLE)患者血清趋化因子CXCL13水平及其受体CXCR5在外周血B细胞、滤泡辅助T细胞(TFH)上的表达,分析CXCL13、TFH细胞水平与疾病活动的相关性,探讨CXCL13及CXCR5受体在SLE发病机制中的可能作用。方法酶联免疫吸附法(ELISA)检测58例SLE患者血浆CXCL13水平,以流式细胞术检测24例SLE患者外周血中CXCR5在CD19+B细胞、TFH细胞上的表达水平。结果 1血清CXCL13SLE患者组为365.96±216.02(pg/ml),高于健康对照组145.66±207.25(pg/ml)(P<0.01)与SLE缓解组167.19±145.37(pg/ml);CXCL13水平与SLE患者的SLEDAI评分、抗核抗体滴度呈正相关(P<0.05)。2SLE患者B细胞CXCR5的表达率为79.03±20.59%,显著低于健康对照组94.25±4.52%,P<0.05。3:SLE活动组TFH细胞水平(%)15.36±7.01高于健康对照组8.92±5.11%,P<0.05;TFH细胞水平与患者的SLEDAI评分呈正相关(P<0.05),与C3、dsDNA无相关性(均P>0.05)。结论 SLE患者外周血CXCL13及表达其受体CXCR5的TFH细胞水平增高,并与病情的活动性相关,提示CXCL13有可能积极参与至SLE的发病机制中,并更有可能为SLE的治疗提供新的靶点。更多还原

【Abstract】 Objective To detect the s levels of chemokine CXCL13 and the expression of receptor CXCR5 on CD19+B cell and CD4+Th cells in patients with systemic lupus erythematosus(SLE),to access the relationship between CXCL13、TFH and SLE disease activity.Methods The serum levels of CXCL13 were measured by enzyme linked immunosorbent assay(ELISA)in 58 patients with SLE and frequencies of CD19+CXCR5Bcells and CD4CXCR5 Tcell w ere analyzed b y flow-cytometry in 24 patients with SLE.Results 1Serum CXCL13 concentration was significantly higher in SLE active patients than inactive SLE group and NC group(P<0.05);Increase in CXCL13 concentration correlated positively and significantly with SLEDAI,Anti-nuclear antibody titers(all P<0.05).2 the frequencies of CXCR5 on CD19+ B cell were significantly down regulated in SLE patients whiling comparing with NC group.3The percentage of CXCR5 in CD4+T cells was increased jn patients with SLE compared with healthy controls,P<0.05.There were positive co-relationship between TFH and SLEDAI.Conclusion Elevated CXCL13 level and increased frequency of circulating Tfh cells in SLE patients are associated with disease activity,indicating CXCL13 and its receptor CXCR5 may play an important role in the pathogenesis of SLE.更多还原