【摘要】 目的了解本地区粘质沙雷菌临床分离株Ⅰ、Ⅱ、Ⅲ类整合子的携带情况,探讨整合子与其耐药表型的相关性。方法收集临床分离的经VITEK-2Compact细菌分析系统鉴定的粘质沙雷菌287株,以煮沸法制备细菌DNA作为PCR扩增模板,采用聚合酶链反应(PCR)检测菌株的第Ⅰ、Ⅱ、Ⅲ类整合子,分析比较整合子阳性菌株与阴性菌株的药敏试验结果差异。结果在287株粘质沙雷菌中,有262株细菌检出Ⅰ类整合子,检出率为91.3%,3株细菌检出Ⅲ类整合子,未检出Ⅱ类整合子。药敏结果显示,第Ⅰ类整合子阳性菌株对环丙沙星和头孢噻肟的耐药率高于Ⅰ类整合子阴性菌株(P均<0.05),对其他抗菌药物的耐药性,Ⅰ类整合子阳性菌株和阴性菌株间差异无统计学意义(P均>0.05)。结论Ⅰ类整合子在粘质沙雷菌中普遍存在,Ⅰ类整合子与粘质沙雷菌的多重耐药无明显的相关性。更多还原

【Abstract】 Objective Understanding of the carrying situation in the region of Serratia marcescens isolatesⅠ,Ⅱ,Ⅲintegron,To explore the relationship between integrons and drug resistance phenotype.Methods Clinical isolates collected by VITEK-2 Compact analysis system for identification of 287 strains of Serratia marcescens.Prepared to boil PCR amplification of bacterial DNA as a template,using the polymerase chain reaction(PCR)testing of classⅠ,Ⅱ,Ⅲintegron.Analysis and comparison the differences of integron positive strains and negative strains in drug sensitive test.Results In 287 strains of Serratia marcescens,262 strains of bacteria detected integron,the detection rate was 91.3%,3 strains of bacteria detected classⅢ integron,II integron were not detected.The drug sensitivity results showed,the classⅠ integron positive strains resistant to ciprofloxacin and cefotaxime were higher than that ofⅠ integron negative strains(P<0.05).Resistance to other antimicrobial drugs,among Ⅰ integron positive and negative strains were no significant differences(P>0.05).Conclusion Ⅰintegron prevalent in Serratia marcescens,classⅠ integron and multidrug-resistant Serratia marcescens is no significant correlation.更多还原