【摘要】 从青海省海北州海晏县同宝村绵羊急性死亡病例中分离到一株B型诺维梭菌,利用该分离菌株(TB05)和B型诺维制苗菌株(C61-4)所产毒素经甲醛灭活,分离株加入氢氧化铝胶、磷酸铝胶及明矾佐剂,制苗株(C61-4)加入磷酸铝胶佐剂制备成羊黑疫单价疫苗,对该疫苗分别进行了安全性检验、家兔免疫试验及血清抗体效价测定试验。结果表明,B型诺维梭菌菌液用5%甲醛72 h即可灭活;家兔皮下注射5 m L该疫苗,观察期内无不良反应,安全性良好。家兔免疫试验表明,分离株氢氧化铝苗、磷酸铝苗及明矾苗0.1 m L免疫组免疫21d后,保护力分别为200、200、100 MLD(家兔最小致死量)、0.5 m L组免疫组保护力均为200 MLD,(C61-4)菌株磷酸铝苗0.1 m L免疫组免疫21d后,保护力低于100 MLD、0.5 m L组免疫组保护力为100 MLD。免疫家兔血清抗体测定结果表明,分离株氢氧化铝苗、磷酸铝苗及明矾苗0.1 m L免疫组免疫21 d后,0.1 m L血清中和力分别为300、200、100 MLD(小鼠最小致死量)、0.5 m L组免疫组0.1 m L血清中和力分别为400、300、300 MLD;(C61-4)磷酸铝苗0.1 m L和0.5 m L免疫组免疫21 d后,0.1 m L血清中和力均为100 MLD。上述试验结果表明,用该地方分离株制备的磷酸铝单价疫苗免疫效果优于C61-4株羊黑疫磷酸铝苗。更多还原

【Abstract】 A Clostridium noviy type B strain was isolated on sheep struck by acute deaths from Haiyan County Haibei.Toxin formthis bacteria and standard strains(C61-4)were inactivated by formaldehyde the isolated strain was adding aluminum hydroxide gel,rubberand aluminum phosphate adjuvant alum,standard strains(C61-4)adding aluminum phosphate gel produced black sheep monovalent vaccine.the vaccine were carried out safety inspection,rabbit immune and serum antibody measurement test. The results showed that the cultureactioned with 5% formaldehyde in37 ℃ for 72 h can be inactivated; there no adverse reactions during the observation period when rabbitsinjected 5 m L of the vaccine,the security is good. Rabbit immune tests showed that 0.1 m L immunity groups of aluminum hydroxide vaccine,aluminum phosphate and alum vaccine the protection force was 200,200,100 MLD(minimum lethal dose in rabbits),0.5 m L protectiveimmunity groups force was 200 MLD,standard strains(C61-4) 0.1ml immunity groups protection was 100 MLD,0.5 m L protective immunitygroups was 100 MLD. Immune rabbit serum antibody test showed that 0.1 m L immunity groups of aluminum hydroxide vaccine,aluminumphosphate and alum vaccine which 0.1 m L serum Can neutralize toxin for 300,200,100 MLD(mouse minimum lethal dose),0.5 m Limmunity groups that 0.1 m L serum Can neutralize toxin for 400,300,300 MLD;standard strains(C61-4)aluminum phosphate vaccine0.1 m L and 0.5 m L immunity groups,which 0.1 m L serum only neutralize toxin for 100 MLD. The experimental results show that theimmunization were batter use the local strain aluminum vaccine than the standard strains.更多还原