【摘要】 本文结合生物信息学资源和生物软件的使用,针对特定的氨基酸序列设计简并引物,利用CODEHOP法设计简并引物克隆鲫鱼肌钙蛋白C(Tn C)基因,并构建系统进化树。证明该基因片段与其他鱼类Tn C基因具有较高的同源性,其中与斑马鱼同源性最高达到96%,与大西洋鲑、白斑狗鱼、虹鳟、斜带石斑鱼、斑点叉尾鮰的同源性分别为92%、92%、91%、91%、85%。研究表明,应用此种方法设计出的引物简并度相对较低,Tm值的修改较为灵活,产物特异性强。更多还原

【Abstract】 We designed degenerate primers for a specific amino acid sequence,combining bioinformatics resources with the biology software. CODEHOP method was used to design degenerate primers to clone carp troponin C(Tn C)gene and to construct phylogenetic trees.This paper proved that the gene fragments showed high homology to other fish Tn C genes and revealed the highest homology of 96% with Danio rerio,and 92%,92%,91%,91%,85% compared with Salmo salar,Esox lucius,Oncorhynchus mykiss,Epinephelus coioides,Ictalurus punctatus homologous respectively.The results indicated that degeneracy rate of these degenerate primers was relatively low,Tm value modification was more flexible,and could be used to obtain specific RT-PCR products.更多还原