【摘要】 参考大鼠Krt71基因序列,用PCR方法对SD大鼠Krt71基因DNA和cDNA序列进行克隆,获得SD大鼠Krt71基因的8 206bp的DNA序列(GenBank登录号:KF311105)和1 575bp的cDNA序列(GenBank登录号:KF303589),编码524个氨基酸。SD大鼠与国外报道的BN大鼠、小家鼠、家猫、家绵羊、家牛、苏门答腊猩猩和人等7个物种的Krt71基因cDNA序列的同源性分别为99.9%,95.2%,88.5%,87.5%,88.0%,88.1%,88.1%;其编码蛋白的氨基酸序列同源性分别为100.0%,98.9%,92.5%,91.5%,91.3%,91.5%,92.1%。这一结果表明Krt71基因在进化过程中具有较高的保守性,但不同物种之间也具有功能上的特异性。通过比较SD大鼠Krt71基因与GenBank数据库中发布的Krt71基因的序列,本试验发现了6个SNP位点,这些位点位于该基因的内含子序列,没有改变氨基酸的性质,这一研究结果为Krt71基因的SNPs数据库提供了新的内容。更多还原

【Abstract】 This study was designed to clone and analyze the DNA and cDNA encoding Krt71 gene from SD rats.The PCR method was developed to clone the Krt71 DNA and cDNA.A full-length DNA sequences of SD rat was 8 206 bp,which has been accepted by GenBank(Accession Number:KF311105),and a full-length cDNA sequences of SD rat were 1 575 bp,which has been accepted by GenBank(Accession Number:KF303589).Krt71 protein encoded by this gene was composed of 524 amino acid residues.The identities of cDNA sequences of Krt71 gene were99.9%,95.2%,88.5%,87.5%,88.0%,88.1% and 88.1% by homologous comparison among SD rat and other species,and in amino acid sequences were 100.0%,98.9%,92.5%,91.5%,91.3%,91.5% and 92.1%,respectively.The result suggested a high degree of conservation of the Krt71 gene among different mammalian taxons.The results derived from information searching by Blast program revealed there were 6SNP sites in the sequences of Krt71 gene DNA between SD rats and that collected in GenBank.These SNP sites were located in the intron sequences,and they did not alter the related amino acids encoded.The results provided news content for SNPs in Krt71 gene.更多还原