【摘要】 近年来,随着功能基因组学的发展,转座子随机突变技术因操作简便易行而被广泛应用。某些具有随机转座特性的转座子及其衍生物,已成为发现新基因、克隆功能基因、发掘已知基因的新功能以及研究蛋白功能的有效工具,其中转座子Tn917、Tn10及mariner类家族转座元件已被改造并成功应用于芽孢杆菌突变体库的构建及基因功能的研究中。本文介绍上述3种转座子的结构、转座特点及在芽孢杆菌中的应用,并分析了mariner类家族转座元件的优势及应用前景。更多还原

【Abstract】 Transposons, useful for random insertional mutagenesis, is able to create random DNA insertions in bacterial chromosomes for discovering new genes, and new functions of known genes. Transposon Tn917, Tn10 and mariner family transposons have been modified and used for constructing transposon insertion library and studying the functions of genes in Bacillaceae successfully. This paper describes the structures, transposition feature and the application in Bacillus spp. of the three transposons. Compared with Tn917 and Tn10, mariner family transposons transpose more randomly and independently of any host factors, making them ideal tools for random mutagenesis in bacteria.更多还原