【摘要】 目的了解北京市市售带壳牡蛎致病性弧菌污染状况。方法 2014年2~11月每月在某水产品批发市场的摊位抽样200只带壳牡蛎,共80份样品(其中腮和肠样品分别为40份)。用常规培养方法检测牡蛎腮和肠(含便)中致病性弧菌,对副溶血性弧菌进行血清学分型,荧光定量PCR检测副溶血性弧菌毒力基因tdh、trh和tlh。结果 80份牡蛎样品中,致病性弧菌阳性样品检出率为62.50%(50/80),副溶血性弧菌阳性菌株检出率为33.75%(27/80),溶藻弧菌阳性菌株检出率为31.25%(25/80);各牡蛎腮和肠样品中,致病性弧菌阳性检出率为67.50%(27/40)和57.50%(23/40);27株副溶血性弧菌共9种血清型;毒力基因检测结果表示,tlh均为阳性,tdh和trh均为阴性。结论北京市市售带壳牡蛎中致病性弧菌污染严重,以副溶血性弧菌和溶藻弧菌检出为主。更多还原

【Abstract】 Objective To investigate the contamination of pathogenic Vibrios in oyster in Beijing markets. Methods Two hundreds of samples were obtained from 80 batches marine products in a wholesale market in Beijing from February to November in 2014. Routine methods were used to detect pathogenic Vibrios in the gills and intestines of oysters. Vibrios parahaemolyticus isolates were serotyped. tdh,trh and tlh genes were detected with real time PCR. Results The isolation rates of pathogenic Vibrios, Vibrio parahaemolytics and Vibrio alginolyticus were 62. 50%,33. 75% and 31. 25%respectively. The isolation rates of pathogenic Vibrio were 67. 50% and 57. 50% in gill and intestine samples respectively.Nine serotypes were identified in the isolated Vibrio parahaemolyticus. tdh and trh gene were negative while trh gene was positive in all 27 Vibrio parahaemolyticus. Conclusion A large proportion of oyster sold in market were contaminated by pathogenic Vibrios and Vibrio parahaemlyticus and Vibrio alginolyticus were the predominant species. Oyster without gills and intestine were safer to consume.更多还原