【摘要】 目的探讨HBV感染慢性肝病患者血清miR-122的表达水平与HBV DNA载量、HBsAg浓度、ALT、AST、AFP水平之间关系,并探讨其临床意义。方法选取慢性HBV携带患者30例（ASC）,慢性乙型肝炎患者（CHB）95例,其中包括轻度30例、中度35例、重度30例。乙型肝炎肝硬化（LC）患者及肝癌（HCC）患者各30例。另选择30例同期健康体检人群血清miR-122含量为基线评价指标。采用实时荧光定量PCR法测定血清miRNA-122含量,通过2^-△△CT法分析其相对表达量。结果血清miR-122相对表达量在健康对照组中最低,与其他各组差异具有统计学意义（P均<0.05）,CHB轻、中、重度3组患者组间比较,重度>中度>轻度,差异具有统计学意义（P均<0.05）;血清miR-122相对表达量与血清ALT、AST、HBsAg水平均呈正相关关系（r=0.894、0.875、0.381,P均<0.01）;肝癌患者血清miR-122相对表达量与AFP呈负相关关系（r=-0.673,P=0.001）;血清miR-122相对表达量与总体lg（HBV DNA）经Pearson相关性分析,两者之间无相关性（r=0.094,P>0.05）。结论血清miR-122水平可能是一种能特异、灵敏地反映肝损伤、评估肝损伤的一种指标,且可以对疾病的炎症活动程度进行评估。血清miR-122与血清AFP呈负相关关系,可能对肝癌的评估、预后及治疗评价具有重要意义。更多还原

【Abstract】 Objectives To explore the relationships between serum miR-122 level and HBV DNA load,levels of HBsAg,ALT,AST and AFP in the serum of patients with chronic liver diseases,and to identify the role of miR-122 as a potential clinical marker.Methods There were 30 healthy control,30 chronic HBV carriers（ASC）;95 chronic hepatitis B（CHB） patients including 30 mild cases,35 moderate cases,30 severe cases;and 30 cases with hepatitis B cirrhosis and 30 cases with hepatocellular carcinoma were selected,respectively.The serum miRNA-122 levels were assessed by real-time fluorescece quantitative PCR(by the 2^-△△CT analysis).Results The relative expression of serum miR-122 level in healthy control group was significantly lower than those of all the other groups（P all < 0.05）.Among the three groups of CHB patients,the serum miR-122 level were severe > moderate > mild,with significant differences（P all< 0.05）.The serum levels of miR-122 and ALT,AST,HBsAg concentration were significantly correlated（r= 0.894,0.875,0.381;P all < 0.01）;the relative expression of miR-122 was negatively correlated with AFP in the serum of patients with hepatocellular carcinoma（r =-0.673,P = 0.001）.By the pearson correlation analysis,there were no correlation between the serum relative expression of miR-122 and the total serum lg（HBV DNA）（r = 0.094,P > 0.05）.Conclusions There was a significant correlation between serum miR-122 level and ALT,AST,indicating that serum miR-122 may be a more specific and sensitive early factor,which maybe an indicator of liver damage,and could be an index to assess the liver inflammation.The serum miR-122 was negatively correlated with the AFP,which indicates that miR-122 may has important significance for evaluation,prognosis and treatment of hepatocellular carcinoma.更多还原