【摘要】 目的:观察金丝桃苷(Hyp)对刀豆蛋白A(Con A)诱导免疫性肝损伤小鼠的保护作用,并探讨其作用机制。方法:昆明种小鼠随机分为6组,分别为正常组,模型组,金丝桃苷低、中、高剂量(12.5,25,50 mg·kg-1)组和联苯双酯(200mg·kg-1)组。给药组连续ig给药10 d,末次给药后1 h,除正常组外,其余各组均尾静脉注射Con A(20 mg·kg-1)造模,造模12 h后,检测血清天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性;肝脏组织中丙二醛(MDA)的含量和超氧化物歧化酶(SOD)的活性;血清白细胞介素-2(IL-2),白细胞介素-4(IL-4),干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)的水平,血中CD3+,CD4+和CD8+含量;并通过HE染色观察肝脏组织的病理学变化。结果:与正常组比较,模型组小鼠血清ALT,AST活性及肝脏组织MDA含量明显升高,肝脏组织SOD活性明显降低,IL-2,IL-4,IFN-γ和TNF-α水平明显升高,CD3+,CD4+和CD8+含量明显降低(P<0.01);与模型组比较,Hyp明显降低免疫性肝损伤小鼠血清ALT和AST活性,降低肝脏组织MDA含量,升高SOD活性,同时明显降低IL-2,IL-4,IFN-γ和TNF-α水平,明显升高CD3+,CD4+和CD8+含量(P<0.05,P<0.01);肝脏病理学检测显示,模型组肝脏组织病理损伤明显,Hyp能明显减轻Con A对肝脏组织的病理损伤。结论:Hyp对小鼠免疫性肝损伤具有一定的保护作用,其机制可能与清除自由基、减少炎性因子释放、调节T细胞亚群平衡有关。更多还原

【Abstract】 Objective: To investigate the protective effect of hyperin( Hyp) on concanavalin A( Con A)-induced immunological liver injury in mice,and to explore its mechanism. Method: Kunming mice were randomly divided into six groups: the normal group,the model group,the low-,middle-,high-dose Hyp groups( 12. 5,25,50 mg·kg- 1) and the bifendate group( 200 mg·kg- 1). The mice in he treated groups were orally administered with hyperin or bifendate for 10 consecutive days. The mice were injected with 20 mg·kg- 1of Con A intravenously except the control 1 hour after the last medication. 12 hours later,the activities of aspartate aminotransferase( AST) and alanine aminotransferase( ALT) in serum,the concentrations of superoxide dismutase( SOD) and methane dicarboxylic aldehyde( MDA) in hepatic tissue,the levels of interleukin-2( IL-2),interleukin-4( IL-4),interferon-γ( IFN-γ) and tumor necrosisfactor-α( TNF-α),CD3+,CD4+and CD8+in blood were determined. In addition,hepatic histopathological examination was also performed. Result: Compared with the normal group,the serum AST and ALT activities,the level of MDA,IL-2,IL-4,IFN-γ and TNF-αincreased,the SOD,T lymphocyte subset CD3+,CD4+and CD8+ratios in hepatic tissue decreased in the model group( P < 0. 01). Compared with model group,the serum AST and ALT activities,the level of MDA,IL-2,IL-4,IFN-γ and TNF-α were reduced,the SOD,T lymphocyte subset CD3+,CD4+and CD8+ratios in hepatic tissue were elevated in the model group( P < 0. 01). Moreover, the pathological injuries of liver tissue were alleviated. Conclusion: Hyp has a potent protective effect against Con A-induced immunological liver injury in mice. The mechanism might be related to removing free radicals,regulating the balance of T cell subgroup and reducing the secretion of inflammation factors.更多还原