【摘要】 目的:建立HPLC测定清热解毒胶囊中绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素含量的方法。方法:采用Eclipse XDB色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.4%磷酸溶液,梯度洗脱,流速0.80 m L·min-1,检测波长275nm。绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素与其相邻质谱峰能完全分离。结果:绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素的线性范围分别为7.83~313 mg·L-1(r=0.999 9),4.30~132 mg·L-1(r=0.999 8),0.836~33.5 mg·L-1(r=0.999 7),4.36~349 mg·L-1(r=1.000 0),0.731~58.4 mg·L-1(r=0.999 7),0.314~12.6 mg·L-1(r=0.999 7)。方法回收率均不低于98%。结论:该方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。更多还原

【Abstract】 Objective: A HPLC method was established for the determination of chlorogenic acid,geniposide,luteolin,baicalin,phillyrin and wogonin in Qingrejiedu capsules. Method: Eclipse XDB column( 4. 6 mm × 150 mm,5 μm) was adopted and eluted in gradient with the mobile phase of acetonitrile-0. 4%phosphoric acid solution. The flow rate was 0. 80 m L ·min- 1. The detection wavelength was set at 275 nm.Chlorogenic acid, geniposide, luteolin, baicalin, phillyrin and wogonin could be completely separated from adjacent mass spectra peaks. Result: The HPLC method showed a good linear range of 7. 83-313 mg·L- 1( r =0. 999 9) for chlorogenic acid,4. 30-132 mg·L- 1( r = 0. 999 8) for geniposide,0. 836-33. 5 mg·L- 1( r =0. 999 7) for luteolin,4. 36-349 mg·L- 1( r = 1. 000 0) for baicalin,0. 731-58. 4 mg·L- 1( r = 0. 999 7) for phillyrin and 0. 314-12. 6 mg·L- 1( r = 0. 999 7) for wogonin,with the recoveries above 98%. Conclusion: The method is simple,accurate and highly reproducible and so can be used to remove interferences from other components and evaluate the quality control of the preparation.更多还原