【摘要】 目的:应用气相色谱-质谱(GC-MS)联用法对淫羊藿挥发油进行指纹图谱研究。方法:采用水蒸气蒸馏法提取巫山淫羊藿和粗毛淫羊藿中的挥发油,利用GC-MS对其化学成分进行分析,通过GC-FID建立了淫羊藿挥发油指纹图谱的分析方法。气相色谱条件为HP-INNOWAX色谱柱,进样口温度260℃,分流比5∶1;程序升温(初始温度60℃,保持10 min,以2℃·min-1升至250℃,保持13 min)。GC-MS参数为柱流速1.0 m L·min-1,MS电子能量70 e V,离子源温度230℃,四级杆温度180℃,传输线温度280℃,全离子扫描,扫描质量范围m/z 40~550,溶剂延迟3 min。GC-FID参数:柱流速1.5 m L·min-1,FID检测器温度260℃,氢气流量30 m L·min-1,空气流量300 m L·min-1,尾吹氮气30 m L·min-1。结果:获得了20份不同产地淫羊藿的指纹图谱峰。结论:该方法的精密度、稳定性和重复性均良好。为淫羊藿药材的质量控制提供了有效手段。更多还原

【Abstract】 Objective:To investigate the chromatographic fingerprints of volatile oil from Epimedii Folium by GC-MS.Method:The volatile oil was extracted from Epimedii Folium by steam distillation method,chromatographic fingerprints of which was detected and calculated by GC-FID,and the mutual fingerprints of ten kinds essential oil from Epimedii Folium was established.GC conditions:HP-INNOWAX column inlet temperature260 ℃,the split ratio of 5∶ 1;temperature program:initial temperature of 60 ℃,keep 10 min,with 2 ℃·min-1rose to 250 ℃,13 min to maintain.GC-MS parameters:column flow rate 1.0 m L·min-1,MS electron energy 70 e V,ion source temperature of 230 ℃,the quadrupole temperature 180 ℃,the transmission line temperature 280℃,full-ion scan mass range m / z 40-550,solvent delay 3 min.GC-FID parameters:column flow rate 1.5 m L·min-1,FID detector temperature 260 ℃,the hydrogen flow rate 30 m L·min-1,the air flow 300 m L·min-1,tail blowing nitrogen 30 m L·min-1.Result:The method was proved to be accuracy,reproducible and stability,by which the ideal fingerprints of volatile oil from Epimedii Folium were obtained.Conclusion:The methods set up are beneficial for the quality control of Epimedii Folium.更多还原