【摘要】 目的:研究紫草素(shikonin)对人宫颈癌Hela细胞增殖、侵袭和迁移能力的影响,并对其机制进行初步探讨。方法:体外培养Hela细胞,分为对照组和紫草素组(浓度分别为4,8,16,32,64μmol·L-1),采用MTT法检测紫草素对Hela细胞增殖的抑制作用;紫草素处理Hela细胞24h后,采用流式细胞术观察细胞凋亡率的改变;Transwell小室法检测细胞侵袭和迁移力的变化;Western blotting法分析黏着斑激酶(FAK)蛋白表达及FAK磷酸化水平的变化;实时荧光定量PCR技术检测FAK mRNA表达的情况。结果:紫草素对Hela细胞的增殖具有抑制作用,且具有浓度和时间依赖性;细胞凋亡率随着紫草素浓度的增加而逐步增高,其中32,64μmol·L-1的紫草素可明显抑制细胞增殖并诱导其凋亡(P<0.001)。4,8,16μmol·L-1的紫草素作用于Hela细胞后,能够显著降低Hela细胞的穿膜细胞数(P<0.05)。紫草素能够呈浓度依赖性的下调Hela细胞中FAK蛋白和mRNA的表达,使FAK磷酸化水平降低。结论:紫草素可显著抑制Hela细胞的增殖、侵袭和迁移能力,其机制可能与抑制FAK磷酸化水平有关。更多还原

【Abstract】 Objective: To investigate the effect of shikonin on proliferation,invasion and migration of human cervical cancer Hela cells and to explore the possible mechanism.Method: The human cervical cancer Hela cells were cultivated and divided into control group and shikonin group(the concentration was respectively 4,8,16,32,64μmol·L-1).The effect of shikonin on cell viability,apoptosis,invasion and migration of cells cultivated after 24h were studied by MTT assay,flow cytometric and Transwell assay,respectively.When cells were cultivated after 24 h,the expression of focal adhesion kinase(FAK) protein,the level of phosphorylated FAK,and mRNA were assessed by Western blot and quantitative real-time PCR analysis.Result: Shikonin inhibited proliferation and induced apoptosis of Hela cells in a dose-dependent manner.When treated with low-toxic doses of shikonin(4,8,16μmol·L-1),the cell invasion and migration were markedly suppressed.Furthermore,shikonin significantly down-regulated the expression of FAK and the level of phosphorylated FAK.In addition,shikonin could increase the expression of FAK mRNA.Conclusion: Shikonin inhibits proliferation and invasion of cervical cancer Hela cells by inhibiting phosphorylation of FAK.更多还原