【摘要】 目的:采用切除双侧卵巢复制骨质疏松症大鼠模型,观察补肾固本方对去势大鼠骨质疏松症骨密度(BMD)、骨矿盐含量及血清降钙素(CT)水平的影响。方法:将105只6月龄雌性Wistar大鼠,随机分为空白组、模型组、仙灵骨葆组(500mg·kg-1)、戊酸雌二醇组(90μg·kg-1)、补肾固本方高、中、低剂量组(16,8,4 g·kg-1)。切除双侧卵巢复制骨质疏松症大鼠模型,造模成功后,给药组ig给予相应药物,给药3个月后取材。双能X射线BMD测量仪及X射线分别测量股骨和腰椎BMD及骨矿盐含量,补肾固本方对股骨及腰椎骨生物力学性能的作用,ELISA法检测CT。结果:与正常组比较,模型组股骨BMD值及骨矿物含量有明显下降(P<0.05),模型组大鼠股骨及腰椎的弹性模量、最大载荷、屈服强度下降(P<0.05),去卵巢组大鼠的血清CT水平明显升高(P<0.01);与模型组比较,各治疗组BMD及骨矿物有所提高(P<0.05,P<0.01),补肾固本方高、中、低剂量组及仙灵骨葆组BMD显著提高(P<0.01),补肾固本方高、中剂量对大鼠腰椎生物力学有一定改善(P<0.05),高剂量组对腰椎弹性模量有明显改善(P<0.01),补肾固本方高、中、低剂量组CT水平较模型组明显改善(P<0.05),其中补肾固本方高剂量组大鼠血清CT水平均明显高于其余各组,中剂量组对CT水平有明显改善(P<0.01),高、中剂量组间比较无差别。结论:补肾固本方能明显改善去卵巢大鼠BMD、骨矿物含量、改善去卵巢大鼠血CT水平,BMD及骨矿物含量明显降低;骨的力学性能得到改善或恢复。更多还原

【Abstract】 Objective: To observe the effect of Bushen Guben decoction( BGD) on bone mineral density( BMD),bone mineral content and serum calcitonin( CT) level in an ovariectomized rat model of osteoporosis. Method: One hundred and five female Wistar rats were randomly divided into the control group,the model group,the Xianlin Gubao group( 500 mg·kg- 1),the estradiol valerate group( 90 μg·kg- 1) and high-,medium-,low-dose BGD groups( 16,8,4 g·kg- 1). The rats except the control group were subjected to bilateral ovariectomy and killed after 3-month treatment. BMD and bone mineral content in the femur and lumbar vertebra,their biomechanical properties were measured by dual-energy X-ray absorptiometry measurement instrument and X-ray. The serum CT level was detected by ELISA method. Result: Compared with the normal group,the BMD and bone mineral content decreased significantly( P < 0. 05),elasticity modulus,maximum loads and yield strength of femur and lumbar vertebra reduced( P < 0. 05),serum CT level declined( P < 0. 01) in the model group. Compared with the model group,the BMD and bone mineral content increased in all treatment groups( P <0. 05,P < 0. 01),the biomechanical properties had good improvement in Xiaoling Gubao group and high-,medium-dose BGD groups( P < 0. 05,P < 0. 01),serum CT level raised in high-,medium-,low-dose BGD groups( P < 0. 05,P < 0. 01). High-dose BGD had better results in the elasticity modulus of lumbar vertebra and CT level than other groups. Conclusion: BGD had good improvement on osteoporosis in ovariectomized rats by affecting BMD and bone mineral content,CT level and biomechanical properties.更多还原