【摘要】 目的:石榴酸(C18∶3)在结构上为顺9,反11,顺13-十八碳三烯酸,为长链烃类,在200~400 nm没有吸收,无法直接用紫外检测器进行检测,本实验采用柱前衍生化方法,使石榴酸带上一个强发色团,使其在200~400 nm有最大吸收,建立石榴籽油中指标性成分石榴酸的柱前衍生化HPLC分析方法。方法:以ω-溴代苯乙酮作为衍生化试剂,以三乙醇胺为相转移催化剂,对石榴籽油进行柱前衍生化,色谱条件采用Diamonsi C18色谱柱(4.6 mm×250 mm,5μm),检测波长272 nm,以甲醇-乙腈-水(68.5∶20∶11.5)为流动相,柱温30℃,流速1.0 m L·min-1。结果:石榴酸在0.026 6~0.133 0 g·L-1(r=0.999)线性关系良好,平均加样回收率为98.7%,RSD 1.8%。结论:该法稳定性、重复性好,定量准确,可作为石榴籽油中石榴酸的定量方法,用于评价和控制石榴籽油的质量。更多还原

【Abstract】 Objective: As the long chain hydrocarbons,punicic acid( C18∶ 3) on the structure of 9( Z),11( E),13( Z)-octadecatrienoic acid,which was in 200-400 nm absorption-free and can’t be measured directly by UV detector. This experiment adopted column derivatization methods,made the punicic acid with a strong chromophore in maximum absorption in 200-400 nm. The determination method of punicicacid in pomegranate seed oil by HPLC was established in this thesis. Method: The punicic acid were derivatized with ω-bromoacetophenone as derivative reagent,triethanolamine as catalyst,the Diamonsi C18column( 4. 6 mm × 250 mm,5 μm) was used. The detection wavelength was set at 272 nm,column temperature 30 ℃,with methanolacetonitrile-water( 68. 5∶ 20∶ 11. 5) aseluent,the flow rate was 1. 0 m L·min- 1. Result: The standard curves of punicic acid is linear within in the range of 0. 026 6-0. 133 0 g·L- 1and the coefficient is 0. 999. The average recovery of punicic acid is 98. 7%,RSD is 1. 8%. Conclusion: The method is reproducible and accurate,which can be used as the determination method of punicic acid inpomegranate seed oil,and used for the quality control of pomegranate seed oil.更多还原