【摘要】 目的探讨汉防己甲素(tetrandrine,TET)对伊曲康唑(itraconazole,ITR)、伏立康唑(voriconazole,VRC)和泊沙康唑(posaconazole,PCZ)体外抗烟曲霉有无增效活性。方法参照M38-A2方案的微量稀释法,以23株烟曲霉临床株为研究对象,测定TET对其的最大非细胞毒性剂量和ITC、VRC、PCZ单独及联合TET对其的最小抑菌浓度(minimal inhibitory concentrations,MICs)。结果 TET抗烟曲霉的最大非细胞毒性剂量为80μg/mL。ITC单独及联合TET对烟曲霉的MIC值范围分别为0.5μg/mL~32μg/mL和0.0625μg/mL~4μg/mL,差异有统计学意义(P<0.05);VRC单独及联合TET的MIC值范围分别为0.25μg/mL~2μg/mL和0.03125μg/mL~0.25μg/mL,差异有统计学意义(P<0.05);PCZ单独及联合TET的MIC值范围分别为0.125μg/mL~1μg/mL和0.03125μg/mL~0.25μg/mL,差异有统计学意义(P<0.05)。结论汉防己甲素在体外对伊曲康唑、伏立康唑和泊沙康唑抗烟曲霉有增效活性。更多还原

【Abstract】 The purpose of this study is to investigate the synergistic effect of itraconazole(ITR),voriconazole(VRC)or posaconazole(PCZ)alone and in combination with tetrandrine(TET)against Aspergillus fumigatus.We investigated the in vitro antifungal effects of ITR/VRC/PCZ alone and in combination with TET against 23 clinical isolates of A.fumigatus using the microdilution method described in M38-A.The minimum inhibitory concentrations(MIC s)of ITR alone and combined with TET against the A.fumigatus were 0.5-32μg/mL and 0.0625-4μg/mL,respectively(P<0.05);the MIC s of VRC alone and combined with TET against the A.fumigatus were 0.25-2μg/mL and 0.031 25-0.25μg/mL,respectively(P<0.05);the MIC s of PCZ alone and combined with TET against the A.fumigatus were 0.125-1μg/mL and 0.031 25-0.25μg/mL,respectively(P<0.05).Thus,TET acts synergistically with ITR/VRC/PCZ against A.fumigatus in vitro.更多还原