【摘要】 目的预测猪附红细胞体(Mycoplasma suis,M.suis)ORF5基因编码蛋白的二级结构及其B细胞抗原表位。方法联合应用生物信息学软件DNAstar与在线分析软件IEDB的B Cell Epitope Prediction Tools预测M.suis ORF5蛋白的二级结构及其亲水性区域、柔韧性区域、抗原指数、表面可及性等结构特征,经综合分析后预测其B细胞优势抗原表位。结果经分析可见,M.suis ORF5蛋白具有较为规则的二级结构,潜在的优势B细胞抗原表位为GGVDGGRD、GMRLPEDSR、EGHPDLESAR氨基酸区段。结论预测出M.suis ORF5蛋白二级结构和B细胞抗原表位,为M.suis免疫原性研究提供新手段,为病原微生物免疫原性研究提供新思路。更多还原

【Abstract】 The objective of the study is to predict the spatial structure and B-cell epitopes of Mycoplasma suis ORF5 protein.The secondary structure,hydrophilicity,flexible region,antigenic index and surface probability were analyzed and predicted by the Protean module in DNAStar software and B Cell Epitope Prediction Tools of IDEB,then B-cell epitopes were predicted by aggregate analysis.Results showed that the secondary structure of Mycoplasma suis ORF5 protein was relatively regular,in which the potential B cell antigenic epitopes were located at GGVDGGRD,GMRLPEDSR,and EGHPDLESAR.The methods of prediction of the secondary structure and B-cell epitopes of Mycoplasma suis ORF5 protein may provide a new method for the study of M.suisimmunogenicity,and provides a new idea for the study on immunogenicity of pathogenic microorganisms.更多还原