Objective:To obtain the recombine exfoliative toxin A(r ETA)by the technique of gene engineering and to provide materials to study the pathogenic mechanism of the exfoliative toxin(ET). Methods:The primers were designed according to the gene sequence of ETA gene announced by Gene Bank. The ETA gene was amplified from the genome of S.aureus and was inserted into the prokaryotic expression vector p QE30. The recombinant plasmid p QE30-ETA was built and was translated into E.coli M15. After the expression cond...
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