【摘要】 目的:探讨高迁移率蛋白B1(HMGB1)促进血管平滑肌细胞(VSMC)增殖与迁移的机制。方法:检测人主动脉血管平滑肌细胞(HA-VSMC)与HMGB1孵育后增殖与迁移的活性、晚期糖基化终产物受体(RAGE)与P38MAPK表达改变,以及RAGE抗体、P38MAPK抑制剂SB203580预处理的影响。结果:HMGB1孵育后,HA-VSMC增殖与迁移活性、RAGE和P38MAPK的表达均明显增加(均P<0.05),且均呈一定的浓度依赖性。用RAGE抗体和SB203580预处理后,HMGB1促进HA-VSMC增殖及迁移的作用均被明显抑制(均P<0.05),RAGE抗体预处理后,HMGB1对P38MAPK表达的诱导作用明显抑制(P<0.05)。结论:HMGB1可能通过与细胞表面RAGE受体结合,激活P38MAPK表达进而促进VSMC的增殖及迁移。更多还原

【Abstract】 Objective:To investigate the mechanism of high mobility of protein B1(HMGB1) in promoting migration and proliferation of vascular smooth muscle cells(VSMCs).Methods:The changes in migration and proliferation ability as well as the expressions of receptor for advanced glycation end-products(RAGE) and P38 MAPK were measured in human aortic VSMCs(HA-VSMCs) after exposure to HMGB1.Further,the influence of RAGE antibody or P38 MAPK inhibitor SB203580 pretreatment was observed.Results:After exposure to HMGB1,the activity of the cell proliferation and migration,as well as the expression of RAGE and P38 MAPK were increased significantly(all P<0.05),and all presented in a concentration-dependent manner.The promoting effects of HMGBl on migration and proliferation ability were significantly inhibited by either RAGE antibody or SB203580 pretreatment(all P<0.05),and HMGB1-induced P38 MAPK expression was significantly inhibited by RAGE antibody pretreatment(P<0.05).Conclusion:HMGB1 can probably promote the migration and proliferation of VSMCs through its binding to cell surface RAGE and then activating P38 MAPK expression.更多还原