【摘要】 为了配制优良单胚杂交组合,避免基因组成重复,提高育种工作效率,利用14对SSR引物对41对甜菜骨干单胚不育系和保持系进行检测。结果表明,14对SSR引物共产生93条扩增带,其中多态性条带76条,平均每对引物扩增多态性条带5.4条,平均多态性百分比为81.7%,平均遗传距离为0.3572,平均遗传相似系数为0.6996。不同品系的平均遗传相似系数为混合(0.7549)>兰45-46(0.7513)>兰71-72(0.7493)>兰19-20(0.7465)>兰85-86(0.7337)。根据UPGMA方法进行聚类,在遗传距离0.20处,可将82份供试材料分为两大类群。结果表明,供试的甜菜单胚雄性不育系和保持系的遗传多样性较丰富,不同品系的不育系和保持系材料之间的遗传基础有差异。更多还原

【Abstract】 In this study, 14 pairs of SSR molecule markers were used to investigate the relationship of 41 pairsof Major Male Sterile and Maintenance Monogerm Sugar Beet Line, in order to prepare the combination of thefine single embryo, avoid the duplication of gene composition and improve the efficiency of breeding. Resultsshowed that, a total of 93 unambiguous bands were obtained, 76 of which were polymorphic. On average, eachpair of primer amplified 5.4 genotypes. The average ratio of polymorphic bands was 81.7%. Compute over-allmean showed that genetic distance was 0.3572, genetic similarity among varieties was 0.6996. The geneticsimilarities within different groups of genotypes were, 0.7549 among mixed one, 0.7513 among LAN 45-46,0.7493 among LAN 71-72, 0.7465 among LAN 19-20, and 0.7337 among LAN 85-86. The 82 genotypes weredivided into two cluster groups based on cluster analysis(at intercept of 0.2). The genotypes from Major MaleSterile and Maintenance Monogerm Sugar Beet Line showed high level of genetic diversity. There was adefinite difference among different Male Sterile and Maintenance Monogerm genetic background.更多还原