【摘要】 采用高效液相色谱法测定不同厂家发酵型茶叶中阿魏酸的含量,色谱条件为Shimpack VP-ODS C₁₈（150 mm×6 mm,5μm）色谱柱,流动相为0.2%冰醋酸-甲醇（28:72）,检测波长314 nm,柱温30℃,流速1.0 mL/min。结果表明,阿魏酸在20¹00μg/mL范围内线性良好（相关系数R²=0.999 8）,平均加样回收率为98.73%,相对标准偏差（RSD）为1.71%。测得9个厂家不同发酵型茶叶中阿魏酸的含量在22.559³0.286μg/g,其中湖南益阳的湘益茯砖茶阿魏酸含量最高,为30.286μg/g。该方法简便快速,适用于发酵型茶叶中阿魏酸含量的测定。更多还原

【Abstract】 The ferulic acid contents of fermented tea from different manufacturers were determined by HPLC.The separation was performed on Shimpack VP-ODS C₁₈ column（150 mm×6 mm,5 μm） with 0.2%glacial acetic acid-methyl alcohol（28:72） as mobile phase.Ferulic acid was detected at314 nm with flowing rate 1.0 ml/min at 30 ℃.The calibration curve was linear（R²=0.999 8） in the range of 20-100 μg/ml for ferulic acid.The average recovery was 98.73%and relative standard deviation was 1.71%,respectively.The ferulic acid contents of fermented tea from 9 manufacturers were 22.559-30.286 μg/g,and the ferulic acid content in Xiangyi brick tea was the highest of 30.286 μg/g.The method was quick and convenient for ferulic acid determination for fermented tea.更多还原