【摘要】 目的:探讨抗痛风胶囊(AGC)对急性痛风性关节炎(AGA)大鼠肿瘤坏死因子(TNF-α)水平的影响。方法:选取雄性SD大鼠60只,随机分为正常组、模型组、秋水仙碱组(0.8 mg/kg)、AGC低剂量组(0.3 g/kg)和AGC高剂量组(1.2g/kg),每组12只,采用关节腔内注射尿酸钠制备AGA模型,检测各组关节软骨、血清及关节液中TNF-α含量,观察大鼠足踝关节肿胀情况等。结果:模型组24 h、48 h步态行为学评分分别为(2.57±0.43)分和(2.11±0.50)分,明显高于其他组(P<0.05);秋水仙碱组、AGC低剂量组和AGC高剂量组24 h、48 h步态行为学评分均较模型组明显降低(P<0.05);模型组在造模2 h后开始肿胀,在12~24 h到达高峰。在造模72 h内各时间段,模型组足踝关节肿胀率均明显高于正常组,差异有统计学意义(P<0.05);秋水仙碱组在12、24和48 h足踝关节肿胀率明显低于模型组(P<0.05),AGC高剂量组在12、24、48和72h足踝关节肿胀率明显低于模型组(P<0.05),AGC低剂量组仅在24 h足踝关节肿胀率低于模型组(P<0.05);模型组关节软骨细胞TNF-α表达较正常组明显上调(P<0.05),而模型组与秋水仙碱组差异无统计学意义(P>0.05);AGC高剂量组和低剂量组关节软骨细胞TNF-α表达较模型组有明显减弱(P<0.05);模型组血清及关节液TNF-α含量均较正常组明显增高(P<0.05);秋水仙碱组、AGC低剂量组和AGC高剂量组血清及关节液TNF-α含量较模型组有所降低(P<0.05);AGC低剂量组和AGC高剂量组血清TNF-α分别为(0.81±0.27)ng/ml和(0.79±0.31)ng/ml,低于秋水仙碱组(P<0.05)。结论:AGC可明显改善AGA大鼠关节症状,降低AGA大鼠关节软骨组织、血清和关节液中TNF-α水平。更多还原

【Abstract】 Objective: To investigate the anti-gout capsule( AGC) effect on acute gouty arthritis( AGA) rat tumor necrosis factor-α( TNF-α) level. Methods: Selected 60 SD rats,were randomly divided into normal group,model group,colchicine group( 0. 8 mg /kg),AGC low dose group( 0. 3 g / kg) and AGC high dose group( 1. 2 g / kg),12 rats in each group,detected each group articular cartilage,serum and synovial fluid TNF-α content,observed rats ankle swelling. Results: The model group 24 h,48 h gait behavior scores were( 2. 57 ± 0. 43) point and( 2. 11 ± 0. 50) point,significantly higher than other groups( P < 0. 05); colchicine group,AGC low dose group and AGC high dose group 24 h,48 h gait behavior score were significantly lower than that of model group( P < 0. 05). The model group began to swell after modeling 2 h,reached the peak at 12- 24 h. The rats in the modeling 72 h each period,the model group ankle swelling rate were significantly higher than the normal group,the difference was statistically significant( P < 0. 05). Colchicine group12 h,24 h and 48 h ankle swelling rate was significantly lower than that of model group( P < 0. 05),AGC high dose group 12 h,24 h,48 h and 72 h ankle swelling rate was significantly lower than that of model group( P < 0. 05),AGC low dose group ankle swelling rate was lower than that of the model group only in 24 h( P < 0. 05). The model group articular cartilage TNF-α expression significantly increased compared with normal group( P < 0. 05),but model group and colchicine group difference were no statistically significant( P >0. 05); AGC high dose group and low dose group articular cartilage TNF-α expression significantly decreased than model group( P <0. 05). The model group serum and synovial fluid TNF-α content were significantly higher than the normal group( P < 0. 05); colchicine group,AGC low dose group and AGC high dose group serum and synovial fluid TNF-α content reduced compared with the model group( P < 0. 05); AGC low dose group and AGC high dose group serum TNF-α were( 0. 81 ± 0. 27) ng/ml and( 0. 79 ± 0. 31) ng/ml,lower than the colchicines group( P < 0. 05). Conclusion: AGC can significantly improve the joint symptoms of AGA rats,decrease AGA rats articular cartilage tissue,serum and synovial fluid TNF-α level.更多还原