【摘要】 目的:探讨microRNA-199a/b-3p抑制乳腺癌细胞运动能力的机制。方法:在乳腺癌MDA-MB-231细胞中,转入miR-199a/b-3p mimcs,免疫印迹法检测PAK4的表达量变化;迁移和侵袭实验检测,超表达miR-199a/b-3p和干扰PAK4表达对乳腺癌迁移侵袭的影响;细胞膜皱起检测,超表达miR-199a/b-3p和干扰PAK4表达对乳腺癌细胞细胞膜皱起的影响。结果:在MDA-MB-231细胞中,超表达miR-199a/b-3p或干涉内源PAK4蛋白表达,均能下调细胞的迁移率、侵袭率和平均细胞膜皱起面积。结论:miR-199a/b-3p抑制乳腺癌细胞运动能力部分是通过抑制PAK4表达实现的,且miR-199a/b-3p具有抗乳腺癌迁移药物开发的潜质。更多还原

【Abstract】 Objective: To analyze the inhibiting mechanism of microRNA-199a/b-3p( miR-199a/b-3p) on cell motility of breast cancer cells. Methods: The expression of PAK4 in MDA-MB-231 cells transfected with miR-199 a / b-3p was detected by Western blot. The biology behaviors of MDA-MB-231 cells transfected with miR-199 a / b-3p or PAK4 SiRNA were analysed by cell migration assay,invasion assay and protrusion dynamics. Results: MiR-199 a / b-3p could suppress the expression of PAK4 in MDA-MB-231 cells. Comparing with normal control,miR-199 a / b-3p or PAK4 SiRNA could suppress the migration,invasion and membrane protrusion of MDA-MB-231 cells. Conclusion: miR-199 a / b-3p could suppress the expression of PAK4,which are considered key breast cancer suppressors and inhibit the cell motility of breast cancer cells.更多还原