【摘要】 目的:探讨Foxp3基因对卵巢癌细胞Skov3侵袭与迁移的影响及作用机制。方法:构建Foxp3基因过表达质粒,采用脂质体转染导入Skov3细胞;以空载体转染细胞株为对照,通过细胞增殖、侵袭、RT-PCR和Western blot实验,观察Skov3细胞Foxp3基因的表达情况,以及过表达Foxp3后细胞的侵袭与转移能力变化,检测MMP-2以及MMP-9基因表达变化。结果:导入过表达Foxp3质粒后,Skov3细胞的Foxp3表达显著升高;与空载体相比,过表达Foxp3基因显著抑制Skov3细胞的侵袭和迁移能力,并显著降低MMP-2和MMP-9蛋白表达水平。结论:上调Fox P3基因表达能通过下调MMP-2和MMP-9基因表达抑制卵巢癌细胞的增殖、侵袭、迁移行为,为深入研究Foxp3基因在体内的抑瘤作用和卵巢癌的基因治疗提供实验依据。更多还原

【Abstract】 Objective: To investigate the effect of Foxp3 on the invasion and metastasis of the ovarian cancer cells Skov3 and the related mechanism. Methods: Plasmid overexpressing Foxp3 gene was constructed and transfected into Skov3 cells using liposome. Proliferation,invasion,RT-PCR and Western blot analysis were done to observe the expression of Foxp3 gene in Skov3 cells and the invasion and metastasis ability. Also,MMP-2 and MMP-9 gene expression was detected. Results: Overexpression of Foxp3 was confirmed in Skov3 cells after Foxp3 plasmid transfection. Compared with the empty vector group,overexpression of Foxp3 gene significantly inhibited the migration and invasion of Skov3 cells. Meanwhile,the expression level of MMP-2 and MMP-9 genes were decreased. Conclusion: Upregulation of Fox P3 gene expression can inhibit cell proliferation,invasion and migration in ovarian cancer Skov3 cells through downregulating MMP-2 and MMP-9 gene expression. This study provided experimental basis for further research on Foxp3 gene and gene therapy for ovarian cancer.更多还原