Objective To establish a rapid and specific real-time fluorescent PCR method for Nam Dinh virus(Ndi V).Methods According to the sequence alignment of Gen Bank and NDi V isolated by our laboratory, we found out the conservative sequence(Rd Rp) and design specific primers and Taq Man- MGB probe. In order to evaluate the reaction system, the concentration of primers and probe were adjusted to optimize the reaction conditions, and the sensitivity,specificity, stability tests for our method were also conducted.R...
【基金】
广东省自然科学基金(2014A030313583)~~
【更新日期】
2015-10-23
【分类号】
R440
【正文快照】
Nam Dinh病毒(NDi V)是一种在我国新发现的昆虫套式病毒。2002年由Nga等[1]从越南急性病毒性脑炎综合征患者的脑脊液中分离出全球首株NDi V。研究者根据病毒的分离地点将其命名为Nam Dinh virus。2013年,中国首次在深圳市龙岗地区的致倦库蚊(Culex pipiens quinquefasciatus)中