【摘要】 目的探讨4种常见阿尔茨海默病(AD)动物模型在研究凋亡机制领域中的优势。方法采用4种常见AD模型(东莨菪碱致记忆障碍模型、鹅膏蕈酸致痴呆大鼠模型、快速老化模型、双转基因小鼠模型),利用Morris水迷宫测试动物模型认知能力;免疫印迹检测Bcl-2蛋白表达水平;TUNEL染色检测凋亡水平。结果在凋亡机制研究探讨中,快速老化小鼠(SAM)在Morris水迷宫测试、Bcl-2蛋白表达以及凋亡检测中其正常组与模型组均有显著差异(P<0.05)。结论 SAM模型在老年痴呆凋亡机制中有明显优势,为中药治疗老年痴呆的临床前研究提供指导。更多还原

【Abstract】 Objective To discuss advantages of 4 common AD animal models on research mechanism of apoptosis. Methods Four common AD model-scopolamine dysmnesia model,ibotenic acid dementia model,senescence accelerated model and double transgenic mice model were used. The cognitive competences of animal models were evaluated by Morris water maze. Bcl-12 protein expression was tested by Western blot and apoptosis was measured by immunohistochemistry TUNEL staining. Results The results of Morris water maze test,Bcl-12 protein expression and apoptosis had significant differences of SAM between normal and model groups( P < 0. 05). Conclusions SAM model has its great advantage in mechanism of apoptosis of AD,so it could offer guidance in preclinical study of treating-AD herbs.更多还原