【摘要】 目的研究赛洛多辛对左氧氟沙星在慢性细菌性前列腺炎大鼠前列腺组织中药动学的影响。方法构建SD大鼠慢性细菌性前列腺炎模型96只,并随机分成对照组(单用左氧氟沙星)和实验组(赛洛多辛和左氧氟沙星联合用药),每组各48只,分别于给药后0.125、0.25、0.5、1、2、4、8和12h每个时间点处死6只模型大鼠,采集两组大鼠的前列腺制作前列腺组织匀浆,高效液相色谱(High performance liquid chromatography,HPLC)测定组织中左氧氟沙星浓度,使用3p97软件计算相关药动学参数。结果对照组和实验组的药-时曲线均符合一室模型。计算出的药动学参数如下:t1/2分别为(4.34±0.25)h和(5.02±0.28)h,tpeak分别为(0.73±0.20)h和(0.86±0.05)h,CL分别为(1.74±0.19)mg/h和(0.64±0.06)mg/h,Cmax分别(3.62±0.3)mg/kg和(8.47±0.51)mg/kg,AUC0~12分别为(21.58±2.00)mg·h/kg和(56.63±4.30)mg·h/kg。结论赛洛多辛能明显提高慢性细菌性前列腺炎模型大鼠前列腺组织中左氧氟沙星的药物浓度。更多还原

【Abstract】 Objective To investigate the influence of silodosin on the pharmacokinetics of levofloxacin in rat’s chronic bacterial prostatitis tissue. Methods A total of ninety-six SD rats were used to develop the models of chronic bacterial prostatitis and then they were divided into two groups randomly: control group(only treated with levofloxacin, n=48) and experimental group(treated with both silodosin and levofloxacin, n=48). Six rats from each group were enthanized to collect their prostate samples to make tissue homogenate at each time point: 0.125, 0.25, 0.5, 1, 2, 4, 8 and 12 h after levofloxacin administration. High performance liquid chromatography(HPLC) was applied to detect the concentrations of levofloxacin and 3p97 software program was used to calculate the pharmacokinetic parameters. Results The levofloxacin concentration-time curves in rats’ prostate of the two groups were both fit the one-compartment model. The major pharmacokinetic parameters of the control group and experimental group as follows: t1/2 were(4.34±0.25)h and(5.02±0.28)h, tpeak were(0.73±0.20)h and(0.86±0.05)h, CL were(1.74±0.19)mg/h and(0.64±0.06)mg/h, Cmax were(3.62±0.3)mg/kg and(8.47±0.51)mg/kg, AUC0~12 were(21.58±2.00)mg·h/kg and(56.63±4.30)mg·h/kg, respectively. Conclusion Silodosin can significantly elevate the concentration of levofloxacin in the prostatic tissues of rats with chronic bacterial prostatitis.更多还原