【摘要】 链霉菌可产生许多具有重要应用价值的次级代谢产物,如抗生素、免疫调节剂、抗肿瘤药物等,它们已经广泛应用于临床医药中。次级代谢产物的生物合成受到转录因子的调控,转录因子通过控制结构基因的转录激活或阻遏解除,决定了表达方式和程度。Lux R家族大ATP结合调控因子(large ATP-binding regulators of the Lux R family,LAL)是一类新的次级代谢途径转录因子,由900~1000个氨基酸残基组成,它的N-端具有Walker A和Walker B模体的ATP/GTP结构域,C-端具有保守的Lux R家族螺旋-转角-螺旋模体的DNA结合域。已在40余种次级代谢产物生物合成基因簇中发现LAL,本文对LAL途径特异性转录调控的研究进展进行综述。更多还原

【Abstract】 Streptomyces produce valuable secondary metabolites, including antibiotics, immunomodulators and antitumor agents. They have been widely used to treat various diseases in clinics. The biosynthesis of the secondary metabolites is regulated by transcriptional regulators, which control expression patterns and levels by activating or repressing the transcription of the structural genes. Large ATP-binding regulators of the Lux R family(LAL) were recently identified to be a novel class of transcriptional regulators in the biosynthetic pathways of the secondary metabolites. The LALs were composed of 900 to 1000 amino-acid residues. The N-terminal ATP/GTP-binding domain was featured with Walker A and B motifs, and the C-terminal DNA-binding domain was represented by a conserved helix-turn-helix motif. They have been found in more than 40 biosynthetic clusters of the secondary metabolites. This review summarized the advances in LAL family pathway-specific transcriptional regulators.更多还原