【摘要】 目的:观察慢性乙型肝炎抗病毒治疗对乙型肝炎病毒X蛋白(hepatitis B virus X protein,HBx)表达的影响。方法:选取96例慢性乙肝初治患者,其中HBe Ag阳性56例,HBe Ag阴性40例。两组患者组内平均分为聚乙二醇干扰素α-2a治疗组(干扰素组)和恩替卡韦治疗组。聚乙二醇干扰素(180μg,每周1次)疗程48周,恩替卡韦(0.5 mg,1次/d)疗程96周。应用蛋白质印迹法检测治疗前和治疗后48周、96周3个时点各组病例肝组织内HBx的表达。结果:所有患者治疗前肝组织HBx均为阳性表达。治疗后48周,HBe Ag阳性干扰素组、恩替卡韦组,以及HBe Ag阴性干扰素组、恩替卡韦组HBx阴性表达率别为73.08%(19/26)、40.74%(11/27)、42.11%(8/19)、45.00%(9/20)。治疗后各组HBx阴性表达率均明显高于治疗前(P均为0.00)。HBe Ag阳性干扰素组HBx阴性表达率明显高于其他3组(P均<0.05)。恩替卡韦治疗96周时,HBe Ag阳性组和HBe Ag阴性组HBx阴性表达率无明显差异[48.15%(13/27)、55.00%(11/20)],和48周时比较也无明显差异(P均>0.05)。结论:干扰素用于HBe Ag阳性的慢性乙肝患者,其抑制X蛋白表达的效果明显优于恩替卡韦。更多还原

【Abstract】 Objective: To investigate the variation of hepatitis B virus X( HBx) protein expression during antiviral treatment for chronic hepatitis B( CHB) patients. Methods: Fifty-six nave CHB patients with HBe Ag-positive and 40 nave CHB patients with HBe Ag-negative were divided randomly into the pegylated-interferon( Peg-IFN) therapy group receiving Peg-IFNα-2a( 180 μg / week) for 48 weeks and entecavir therapy group receiving entecavir( 0. 5 mg / d) for 96 weeks. All patients were tested hepatic tissue for the HBx protein expression levels by Western-blotting method at treatment weeks 0,48,and 96.Results: At week 0,The HBx protein expression of all patients were positive. At week 48,the rates of the HBx protein negative expression in Peg-IFNα-2a therapy group and entecavir therapy group with HBe Ag-positive,and in Peg-IFNα-2a therapy group and entecavir therapy group with HBe Ag-negative,were 73. 08%( 19 /26),40. 74%( 11 /27),42. 11%( 8 /19),45. 00%( 9 /20),and were significantly different from at week 0( all P values were 0. 00); then,the rate in Peg-IFNα-2a therapy group was significant difference from others three groups( all P values were less than 0. 05). At week 96,the rates of the HBx protein negative expression had no significant difference in entecavir therapy group with HBe Ag-positive and with HBe Ag-negative[48. 15%( 13 /27),55. 00%( 11 /20) ]; and compared at week 48,the results were similar( All P values were greater than 0. 05). Conclusion: Compared to entecavir,Peg-IFN was more potent to suppress the HBx protein expression.更多还原