【摘要】 目的探讨去甲斑蝥素对人胰腺癌PANC-1细胞凋亡的作用及其机制。方法将PANC-1细胞株随机分为处理组和对照组,处理组加入不同浓度的去甲斑蝥素培养24h后,CCK-8法检测细胞增殖情况;流式细胞术分析细胞的凋亡情况;免疫印迹法检测细胞内质网应激和凋亡相关蛋白的表达;荧光定量PCR技术检测细胞内质网应激和凋亡相关蛋白mRNA表达。结果不同浓度去甲斑蝥素处理PANC-1细胞24h后,与对照组相比,能降低细胞的存活率并诱导其凋亡。与对照组相比,处理组中内质网应激和凋亡相关蛋白的表达水平均上调(均P<0.05),同时其mRNA的表达水平亦均上调(均P<0.05)。结论去甲斑蝥素能明显抑制人胰腺癌PANC-1细胞的生长并诱导其凋亡,并且具有浓度依赖性,这一作用可能是通过内质网应激介导的凋亡途径实现的。更多还原

【Abstract】 Objective To investigate the effect of norcantharidin on human pancreatic cancer PANC-1 cells.Methods PANC-1 cells were cultured with 0.1%DMSO and norcantharidin(0,10,50,75,100,150,200μmol/L) for 24 h.The growth inhibitory effect was observed by using CCK-8,cell apoptosis was determined by flow cytometry(FCM).The expressions of endoplasmic reticulum stress(ERS) markers were detected by Western blot,expressions of GRP78 and GADD153 mRNA were detected by real-time PCR after exposure to norcantharidin.Results CCK-8 assay showed that norcantharidin significant inhibited the growth of PANC-1 cells.Apoptosis rates of PANC-1 cells treated with norcantharidin were significantly higher than those of control group.Western blot analysis showed that norcantharidin up-regulated ERS markers(P<0.05).Real-time PCR showed that the expression of GRP78 and GADD153 mRNA in PANC-1 cells treated with norcantharidin was higher than that of control group(P<0.05).Conclusion Norcantharidin can inhibit the growth of human pancreatic cancer PANC-1 cells and induce cell apoptosis.ERS-mediated apoptosis pathway may be involved in norcantharidin-induced apoptosis in pancreatic cancer PANC-1 cells.更多还原