【摘要】 目的:探讨神经元特异性烯醇化酶(NSE)和突触素(SYN)蛋白在人胚胎食管组织不同发育阶段的分布特征。方法:经伦理委员会批准,收集受精龄为7~16周的人胚胎标本16例,应用免疫组织化学染色法检测胚胎食管组织内NSE和SYN的表达,定量分析其变化规律。结果:第2~4个月胎龄时,肌间神经丛和黏膜下神经丛内细胞呈NSE阳性表达,阳性表达的数量和强度第3个月胎龄甚于第2个月胎龄,第4个月胎龄甚于第3个月胎龄。仅在第3个月胎龄时,食管黏膜上皮层内部分细胞呈NSE阳性表达。第2~4个月胎龄时,SYN在食管各层组织内均呈广泛阳性或强阳性表达,尤其在肌间神经丛和黏膜下神经丛更为明显。结论:在人胚胎食管发育阶段,NSE和SYN均参与食管壁神经系统的发育过程。更多还原

【Abstract】 Objective:To investigate the expression of neuron-specific enolase(NSE) and synaptophysin(SYN) proteins in different developmental stages of human embryonic esophagus.Methods;Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2,3and 4 month gestational age(n = 16).One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age.Results:In fetuses with 2,3 and 4months of gestation,the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38 ±8.37,25.00 ± 11.54 and 38.00 ± 15.09,respectively;the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38 ± 14.93,62.25 ± 18.59 and 56.44 ± 14.70,respectively.NSE-positive cells were detected in the esophageal epithelium only at the third month.In the fetuses at 2,3 and 4 months of gestation,SYN in all layers of esophageal tissue were positively or strong positively expressed,especially in the myenteric plexus and submucosal plexus.The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2,3 and 4 month gestation were 54.69 ± 9.34,51.84 ±6.10 and 46.41 ±6.44,respectively.Conclusion:SYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development.更多还原