【摘要】 目的开发制备用于体外培养小鼠胰岛细胞团用的鼠尾胶。方法剥离大鼠鼠尾制作鼠尾胶原,并用该胶原在不同条件下培养小鼠胰岛细胞团,用FD-PI染色观察胰岛活性。结果用自制鼠尾胶原培养胰岛细胞团24 h后,细胞团依然圆整、活性好。结论用本法制备的鼠尾胶可用于小鼠胰岛细胞团的培养,胰岛细胞团在该鼠尾胶中可保持活性。更多还原

【Abstract】 Objective:Exploit and make the rattail collagen used for culturing the mice islet cells in vitro.Process: Stripping the rat’s tail to make rattail collagen,and use this collagen to culture mice islet cells under the different conditions, with FD-PI staining to observe the islet activity.Result: After 24 hours, the cultivation of islet cells with the self-control rattail collagen, the cell mass is still round and has good activity.Conclusion: The rattail collagen made with this method can be used into the cultivation of mice islet cell mass, the islet cell mass can keep activity in this rattail collagen.更多还原