【摘要】 目的:观察电针对脑缺血再灌注模型大鼠血清白介素-6(interleukin-6,IL-6)、IL-8和IL-10的影响,探讨电针防治缺血性脑病的作用机理。方法:雄性Sprague Dawley(SD)大鼠按随机数字表分为假手术组、模型组和电针组,以上三组又各分为6 h和24 h两个亚组。假手术组仅予手术血管分离,插入线栓,不进行治疗。模型组及电针组采用改良Longa线栓法复制大脑中动脉局灶性脑缺血再灌注模型。模型组仅造模,不作任何治疗;电针组治疗取百会(GV 20)及大椎(GV 14),采用电针疏密波刺激30 min。应用Elisa法测定各组大鼠IL-6、IL-8和IL-10水平。结果:脑缺血再灌注后6 h,模型组血清IL-6、IL-8及IL-10水平均较假手术组高(P<0.01,P<0.05,P<0.05);电针组血清IL-8水平低于模型组(P<0.05),血清IL-6及IL-10水平与模型组无统计学差异。脑缺血再灌注后24 h,电针组血清IL-6及IL-8水平低于模型组(均P<0.05),血清IL-10水平三组比较均无统计学差异。结论:电针早期介入脑缺血损伤可以调节血清IL-6、IL-8水平。更多还原

【Abstract】 Objective: To observe the effect of electroacupuncture(EA) on serum interleukin(IL)-6, IL-8 and IL-10 in rat models of cerebral ischemia-reperfusion, and to discover the mechanism of EA in preventing and treating cerebral ischemia. Methods: Male Sprague Dawley(SD) rats were randomized into a sham-operation(SO) group, a model control(MC) group, and an EA group, which were sub-grouped into a 6-hour group and a 24-hour group. In the SO group, rats only received vessel separation with filament placed inside without any treatment. In the MC and EA groups, the focal cerebral ischemia-reperfusion model was induced by using modified Longa method with intraluminal filament. The MC group didn’t receive any treatment; the EA group received EA at Baihui(GV 20) and Dazhui(GV 14) with sparse-dense wave for 30 min. The levels of serum IL-6, IL-8 and IL-10 were detected by using Elisa test. Results: Six hours after ischemia-reperfusion injury, the levels of serum IL-6, IL-8 and IL-10 in the MC group were significantly higher than those in the SO group(P<0.01, P<0.05, P<0.05); the level of serum IL-8 in the EA group was significantly lower than that in the MC group(P<0.05), while there were no significant differences in comparing IL-6 and IL-10 between the EA group and the MC group. Twenty-four hours after ischemia-reperfusion injury, the levels of serum IL-6 and IL-8 in the EA group were significantly lower than those in the MC group(both P<0.05), while there were no significant differences in comparing the level of IL-10 among the three groups. Conclusion: Early intervention by EA can regulate the levels of serum IL-6 and IL-8 in cerebral ischemic injury.更多还原