【摘要】 目的利用正交试验研究铁线透骨草中槲皮素的最佳提取条件,测定不同批次铁线透骨草中槲皮素的含量。方法设置L9(34)正交试验,安排提取温度、溶媒(甲醇)量、提取时间、加盐酸量4个因素,HPLC法检测;色谱柱:Hypersil C18色谱柱(4.6mm×250mm,5μm);流动相:甲醇-0.1%乙酸(50∶50);流速:1.0mL·min-1;柱温:25℃;检测波长:375nm。结果最佳提取条件为:样品量约2.0g,加入35mL甲醇、10mL浓盐酸,浸泡0.5h,于40℃超声震荡提取40min。在此条件下测定的8个批次铁线透骨草中槲皮素的含量为0.109 9~0.880 3mg·g-1。结论提取方法效率高、时间短、温度低,是提取铁线透骨草中槲皮素较好的方法。不同批次铁线透骨草中槲皮素的含量相差较大。更多还原

【Abstract】 Objective To study the optimal extraction conditions of quercetin fromClematis intricata Bunge by orthogonal test and determine contents of quercetin in different batches of Clematis intricata Bunge.Methods Setting the L9(34)orthogonal test table,arranging four factors:extraction temperature,amount of solvent(methanol),extraction time and amount of hydrochloric acid.Detecting by HPLC;column:Hypersil C18(5μm,4.6 mm×250 mm),mobile phase:CH3OH-0.1% CH3COOH(50∶50),flow rate:1.0 mL/min,column temperature:25 ℃;determining wavelength:375nm.Results The optimal condition was that the sample with about 2.0g conical flask with plug was poured35 mL CH3OH and 10 mL concentrated hydrochloric acid,then was soaked about 0.5h,and extraced40 min at the temperature of 40 ℃ with ultrasound concussion.Conclusion The extraction method could have high efficiency,short time and low temperature,and be better to extract quercetin from Clematis Intricata Bunge.The contents of quercetin could differ significantly in different batches of Clematis Intricata Bunge.更多还原