【摘要】 目的:分离并初步筛选瓜蒂催吐作用的有效部位。方法:以80%乙醇回流提取制得的瓜蒂总提取物后,用硅胶柱溶剂极性依次递增分离法(石油醚-乙酸乙酯-甲醇连续洗脱)制得的分段提取物(石油醚Ⅰ、乙酸乙酯Ⅱ、甲醇Ⅲ)为药效研究对象,筛选对犬致吐作用有活性的有效部位,最后通过特殊颜色反应确定有效部位的化学性质。结果:瓜蒂中乙酸乙酯提取物具有催吐作用,与蒸馏水组相比差异有显著性(P<0.01)。颜色反应说明该部位主要含有葫芦素等物质。结论:瓜蒂催吐作用有效部位可能是总葫芦素类。更多还原

【Abstract】 Objective:To separate and screen the effective fractions of the muskmelon fruit pedicel. Methods:With 80% ethanol extract obtained after the muskmelon fruit pedicel total extract by silica gel column in ascending order of solvent polarity separation(petroleum etherethyl acetate- methanol continuous elution) obtained sub-extract(petroleum ether Ⅰ,ethyl acetate Ⅱ,methanol Ⅲ) to study the effective part capable of inducing vomiting in dogs. Special color reaction was applied to confirm the chemical property of the effective fractions of the muskmelon fruit pedicel. Results:The ethyl acetate extract of the muskmelon fruit pedicel has the emetic effects compared with the control group(P<0.01). The effective fractions contained Cucurbitacin substances identified by special color reaction. Conclusion: Cucurbitacin substances might be the emetic fraction in the muskmelon fruit pedicel.更多还原