Construction,expression,and identification of a fusion gene from an outer membrane protein(peb1A)gene of Campylobacter jejuni and the heat-labile enterotoxin B subunit(ltB)gene of Escherichia coli
Objectives To use overlapping PCR to construct a prokaryotic expression system for expression of the fusion gene ltB-peb1 Afrom the heat-labile enterotoxin B subunit(ltB)gene of Escherichia coli and an outer membrane protein(peb1A)gene of Campylobacter jejuni,to induce its expression,and to identify that gene. Methods The genes ltB and peb1 A were amplified with PCR.The ltB gene was fused with the peb1 Agene using overlapping PCR and the fusion gene was inserted into a pET-28a(+)prokaryotic expression vecto...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
