Objectives To detect differences in transcription of the hypoxia-inducible factor-1(hif-1)gene from adult male and female Ascaris suumand to examine molecular mechanisms for sex differences in A.suumin order to provide information for control of ascariasis. Methods Total RNA was extracted from male and female adult A.suumand cDNA was synthesized by reverse transcription.Adult A.suumof different sexes were examined for transcription of the hif-1αand hif-1βgenes using real-time PCR.The actin gene served as a ...
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