Objective In molecular biology experiments,expression vectors are usually constructed in order to stably express foreign genes in cells.In this study,two different methodologies were used to generate pMIR-reporter vectors.Methods In conventional double enzyme restriction,PCR fragments of the target gene were first inserted into a T-A cloning vector.The restriction enzymes HindⅢ and MluⅠ were then used to generate sticky ends on a linearized pMIRreporter vector and PCR fragments of a target gene from a T-A c...
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