【摘要】 目的探讨溶血磷脂酸(LPA)协同转化生长因子1(TGFβ1)的促上皮间质转化(EMT)作用。方法观察TGFβ1及LPA诱导SW480细胞后细胞形态变化,Western blotting方法检测E-Cadherin、Vimentin的表达变化以及细胞增殖活性变化。结果 TGFβ1及LPA均能使SW480细胞形态发生EMT改变;两者联合诱导细胞EMT改变更为明显。空白对照组、LPA组、TGFβ1组、LPA组+TGFβ1组,E-Cadherin表达依次下降;Vimentin表达未见明显差异。结论 LPA可能协同TGF-β1产生EMT作用,下调LPA的表达或可抑制肿瘤侵袭转移。更多还原

【Abstract】 Objective This study aimed to investigate whether LPA could synergize TGFβ1 to increase epithelial-to-mesenchymal transition( EMT). Methods Western blotting was used to examine the EMT markers: E-cadherin and Vimentin. MTT was used to examine the cell proliferation of 5-Fu and LOHP with or without LPA. Western blotting was used to examine the apoptosis markers Bcl-2,Bax and Caspese-3after LPA treatment. Results TGFβ1 and LPA induced the morphology change of colorectal cancer cell line SW480,and combination group LPA / TGFβ1 induced more significant change. Compared with the LPA group,the difference of E-cadherin in blank control group 1% FBS,and TGFβ1 treatment group,the LPA /TGFβ1 co-treatment group were significant,while the difference of Vimentin was not significant. Conclusion LPA synergizes with TGF-β to play an important role in EMT. Inhibition of LPA may decrease the invasion and migration of colon cancer cells.更多还原