【摘要】 目的研究通络驻景丸对糖尿病代谢记忆的抗氧化机理。方法链脲佐菌素(STZ)诱导SD大鼠糖尿病,造模成功的大鼠分成6组:糖尿病模型组、代谢记忆模型组、阳性对照组和通络驻景丸大、中、小剂量组,另设一组不造模的空白对照组。空白对照组、糖尿病模型组、代谢记忆模型组分别灌胃给予蒸馏水,阳性对照组灌胃给予羟苯磺酸钙,通络驻景丸大、中、小剂量组分别灌胃给予通络驻景丸不同剂量。每日给药1次,容积10ml/kg,连续6个月。从第4个月开始除空白对照组和糖尿病模型组,其余5组给予胰岛素控制血糖至正常。实验期间每周检测血糖1次,6个月后检测各组大鼠血清中的超氧化物歧化酶(SOD)和谷胱甘肽氧化物酶(GSH-Px)的活性及丙二醛(MDA)含量。结果与正常对照组比,糖尿病模型组和代谢记忆模型组大鼠血清SOD和GSH-Px活性降低,MDA含量升高;通络驻景丸中、高剂量组能够显著提高模型大鼠血清SOD和GSH-Px的活性,显著降低MDA含量。结论通络驻景丸具有抗氧化作用,能够逆转代谢记忆所致的氧化应激反应,阻止糖尿病视网膜病变的持续发生发展。更多还原

【Abstract】 Objective To study the effect of Tongluozhujing pill(TLZJP) on antioxidant mechanism of metabolic memory in diabetic retinopathy. Methods SD rats were induced by STZ, after model succeeded, all rats were randomly divided into 6 groups: diabetes model group(DM), metabolic memory model group(MM), positive control group(PC) and Tongluozhujing pill low dose group(TZPL), middle dose group(TZPM), high dose group(TZPH). Another group was normal control group(NC). NC group, DM group and MM group were administrated with distilled water,PC group were administrated with calcium dobesilate,TZPL, TZPM, TZPH group were given TLZJP different dose(10ml/kg, once per day, intragastrically) respectively, for 6 months. From the fourth month, except for NC and DM group, the others were treated with insulin to control blood glucose normally. The blood glucose was detected once a week, the serum levels of SOD, GSH-Px and MDA were detected after6 months. Results Compared with NC group, activity of SOD and GSH-Px was lower in DM group and MM group, MDA levels was higher. TZPM and TZPH groups could increase the activity of SOD and GSH-Px, decrease the level of MDA significantly. Conclusion TLZJP has antioxidant effect, which can reverse the oxidative stress induced by metabolic memory, and prevent the development of diabetic retinopathy.更多还原