【摘要】 以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型,灌胃给予高、中、低剂量(0.4、0.2和0.1 g/kg)蚯蚓冻干粉,每天1次,同时饲喂高脂饲料,共饲养10周。提取小鼠肝脏总RNA,应用反转录基因扩增(RT-PCR)技术检测羟甲基戊二酸单辅酶A还原酶(HMG-CoAR)信使核糖核酸(mRNA)水平。结果表明,试验组小鼠HMG-CoAR mRNA相对含量显著低于高脂饲料组小鼠,提示蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平,可能与下调肝HMG-CoAR mRNA表达有关。更多还原

【Abstract】 Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemic mouse models The mice were treated with earthworm lyophilized powder at dosages of 0.4,0.2 and 0.1 g/kg once a day,meanwhil they were fed with high-level lipid feeds for 10 weeks.The total RNA of liver were extracted,and the changes of live hydroxymethylglutaryl CoA reductase(HMG-CoAR) gene expression were analyzed by RT-PCR.The results indicate that earthworm lyophilized powder down-regulated HMG-CoAR mRNA expression markedly,suggesting that the effec of earthworm lyophilized powder on reducing serum cholesterol in hyperlipidemic mice was probably involved in down regulating HMG-CoAR mRNA expression.更多还原