【摘要】 目的探讨长期使用糖皮质激素对大鼠的骨质量影响。方法 3月龄SD大鼠共38只,雌性。随机分成4组,分别是:(1)基础对照组(B组,8只,实验开始时处死,建立本批大鼠底线);(2)正常对照组(C组,10只,腹腔注射生理盐水75 mg/kg);(3)低剂量激素组(L组,10只,腹腔注射甲强龙50 mg/kg);(4)高剂量激素组(H组,10只,腹腔注射甲强龙75 mg/kg)。实验开始时,低剂量激素组和高剂量激素组连续使用甲强龙3天(75 mg/kg·d),停用一周后,按计划使用甲强龙,每周一次,用药24周后取股骨和第三腰椎行骨生物力学实验。结果 (1)与正常对照组相比,低剂量激素组和高剂量激素组的实验后体重明显减少(P<0.05);(2)与正常对照组相比,低剂量激素组和高剂量激素组股骨的结构力学参数(载荷、刚性)和材料力学参数(破断强度、刚性模量)均显著降低(P<0.05);(3)与正常对照组相比,低剂量激素组和高剂量激素组腰椎的材料力学参数(破断强度、刚性模量)均显著降低(P<0.05)。结论长期使用糖皮质激素激素,无论剂量高低,均会导致大鼠股骨和腰椎骨质量下降。更多还原

【Abstract】 Obje ctive To determine the effect of long-term use of methylprednisolone on bone biomechanics of rats. Me thods Thirty-eight 3-month-old female SD rats were chosen. They were randomly divided into basal control group( B group,8 rats were sacrificed at the beginning of the experiment as baseline),normal control group( C group,10 rats were given intraperitoneal injection of 75 mg / kg normal saline),lowdose methylprednisolone group( L group,10 rats were given intraperitoneal injection of50 mg / kg methylprednisolone),and high dose methylprednisolone groug( H group,10 rats were given intraperitoneal injection of75 mg / kg methylprednisolone). Methylprednisolone was given in L group and H group for 3 days,and stopped for a week. Then the intraperitoneal injection was given once a week according to the plan. After 24 weeks of the treatment,the femurs and the lumbar spine L3 were collected for biomechanical experiment. Re sults( 1) Compared to that in C group,the body weight in L group and H group decreased obviously after the experiment( P < 0. 05).( 2) Compared to those in C group,structural mechanical parameters( load and rigidity) and material mechanical parameters( breaking strength and modulus of rigidity) of the femurs in L group and H group significantly decreased( P < 0. 05).( 3) Compared to those in C group,structural mechanical parameters( load and rigidity) and material mechanical parameters( breaking strength and modulus of rigidity) of the L3 in L group and H group significantly decreased( P < 0. 05). Conclusion The long-term use of methylprednisolone,regardless of dose,results in the decrease of bone quality of the femur and the lumbar spine of rats.更多还原