【摘要】 目的:研究PKM2在非小细胞肺癌组织中的表达及其与放疗敏感性的关系。方法:选取2011年5月至2013年10月四川省人民医院非小细胞肺癌120例患者,术后进行2个月的放射治疗,按照疗效将患者分为4组。RT-PCR检测患者放疗前手术切除的肿瘤组织和癌旁组织中PKM2 m RNA表达水平。Western blot和免疫组织化学检测肿瘤组织和癌旁组织中PKM2蛋白表达。RT-PCR和Western blot方法检测不同疗效组PKM2表达水平。结果:非小细胞肺癌放疗2个月后,治疗有效率为49.2%。PKM2在非小细胞肺癌癌组织中表达显著高于相应的癌旁组织。PKM2表达水平与患者放疗疗效呈负相关。结论:PKM2在非小细胞肺癌中表达高于癌旁组织,PKM2低表达患者对放疗敏感性高。更多还原

【Abstract】 Objective:To investigate the expression level of pyruvate kinase M2(PKM2) in tissues of non-small cell lung cancer(NSCLC) patients and the correlation between PKM2 expression level and radiation sensitivity.Methods:A total of 45 NSCLC patients were chosen and treated with radiotherapy for two months after surgery.The patients were classified into four groups based on the curative effect.The m RNA expression levels of PKM2 in tumor and the homologous paraneoplastic tissues of NSCLC patients were detected prior to radiotherapy using real time-polymerase chain reaction(RT-PCR),and the protein expressions of PKM2 in the tumor and paraneoplastic tissues of NSCLC patients were detected with Western blot analysis and immunohistochemical method.The m RNA and protein expression levels of PKM2 in the tumor tissues of different groups were detected with RT-PCR and Western blot assays.Results:The effective rate of radiotherapy for 2 months is 44.8% in NSCLC patients.The expression level of PKM2 is significantly higher in tumor tissues than in homologous paraneoplastic tissues of NSCLC patients and is negatively correlated with the curative effect of radiotherapy.Conclusion:The expression level of PKM2 is significantly higher in tumor tissues than in the paraneoplastic tissues of NSCLC patients.Patients with lower PKM2 expression level are more sensitive to radiotherapy.更多还原