【摘要】 目的:研究热休克蛋白27(HSP27)在三阴性乳腺癌(TNBC)中的表达与乳腺癌临床、病理指标之间的关系,探讨HSP27与细胞凋亡系统脂肪酸合成酶/脂肪酸合成酶配体(FAS/FASL)之间的相关性及其在TNBC侵袭转移中的作用。方法:采用免疫组织化学S-P法检测100例TNBC、100例非TNBC及50例癌旁组织中HSP27及FAS/FASL表达,对HSP27表达与TNBC临床及病理指标的相关性以及HSP27与FAS/FASL之间表达的相关性进行分析。结果:HSP27在TNBC中表达显著高于非TNBC及癌旁组织(P<0.05),FAS/FASL在TNBC与非TNBC及癌旁组织中的表达差异具有统计学意义(P<0.05)。HSP27与FAS的表达呈负相关(P<0.05),HSP27与FASL的表达呈正相关(P<0.05),FAS与FASL的表达呈负相关(P<0.05)。HSP27在TNBC中表达与不同年龄、分期、肿瘤直径无相关性(P>0.05),与有淋巴结转移、淋巴结转移枚数、P53、Ki-67的表达相关(P<0.05)。结论:HSP27过表达与FAS/FASL系统表达失调可能促进TNBC细胞增殖侵袭及转移,导致不良预后。更多还原

【Abstract】 Objective: To study the relationship between the expression of heat shock protein 27(HSP27) in triple negative breast cancer(TNBC) and the clinico-pathological indexes of breast cancer, investigate the correlation between HSP27 and the fatty acid synthetase(FAS)/fatty acid synthetase ligand(FASL) of the cell apoptosis system in the Fas/Fasl system, and study the role of HSP27 in the invasion and metastasis of TNBC. Methods: The immunohistochemical S-P method was used to detect the expression of HSP27and(FAS)/(FASL) in 100 TNBS tissue sampres, 100 non-TNBS tissue sampres, and 50 paraneoplastic tissues. This method was also used to analyze the correlations between the expression of HSP27 and the clinical and pathological indexes of TNBC, as well as between the HSP27 expression and FAS/FASL expression. Results: HSP27 expression was significantly higher in TNBC than in the non-TNBC and paraneoplastic tissues(P<0.05). Statistically significant differences were observed in the FAS/FASL expression in the TNBC, non-TNBC, and paraneoplastic tissues(P<0.05). HSP27 expression had a negative correlation with FAS expression(P<0.05).HSP27 expression was positively correlated with FASL expression(P<0.05). FAS expression had a negative correlation with FASL expression(P<0.05). HSP27 expression in TNBC was not correlated with age, staging, and tumor size(P>0.05), whereas HSP27 expression was correlated with lymph node metastasis, number of nodal metastases, and P53 and Ki67 expression(P<0.05). Conclusion: The overexpression of HSP27 and the expression dysregulation of the FAS/FASL system may play a role in promoting TNBC transfer and invasion, cell proliferation, and poor prognosis.更多还原